The Use Of Positive And Negative Controls To Determine The Macromolecules Present In An Unknown Solution

unravel # 6130, Wednesday, 7-10 PM, B2-151 Lab performed: September 21st, 2011 Introduction E accurate living thing is dependent on large confused molecules, cognize as macromolecules. The objective of this research laboratory was to justly bring out which macromolecules the unknown solution was comprised of using diverse substances as experimental secures. There are four major types of biologic macromolecules - carbohydrates, lipids, proteins, and nucleic acids - made up of elements such as carbon, hydrogen, oxygen, nitrogen, sulfur, and match in various combinations. Macromolecules are essential for survival; they show morphological support, a source of stored fuel, the ability to store and obtain hereditary information, as well as the ability to further up biochemical reactions; hence their importance in biological systems is notable. (Prakash, 2008). The control samples have a known outcome; in this lab both incontrovert ible and disallow controls were used to determine the personal identity of the unknown solution. The macromolecules being well-tried in this lab were carbohydrates (monosaccharides and polysaccharides) and proteins. To identify the figurehead of these macromolecules in a substance, three different fundament races were performed. Lugols iodine solution was used for acknowledgment of starch and animal starch - polysaccharides - in the twelve solutions. A positive outcome of the judge results in a colour reposition; luscious-black in the presence of starch and a red-brown in the presence of glycogen. A negative outcome results in no colour change over and all solutions remain a very pale discolor (Pavia, 2005). Benedicts solution was used to identify the presence of reducing sugars; the aldehyde running(a) group is the part that reacts in the test. A positive indicant of the test is the formation of a benighted precipitate of the blue solution, ranging from yellow-green to red-brown (Hequet and Abidi, 2006). Lastl! y, the Biuret solution was used to test for the presence of... If you expect to get a full essay, sound out it on our website: BestEssayCheap.com

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